Technology /
separation principle |
Silica-membrane technology /
chaotropic salt binding |
| Material |
Silica membrane
|
| Format |
Spin columns |
Low throughput systems, from extra small to extra large scale |
|
8-well strips
|
Medium-throughput systems,
for vacuum manifolds, centrifuges, and robotic systems
|
|
96-well plates |
High-throughput systems,
for vacuum manifolds, centrifuges, and robotic systems
|
| Procedure |
Principle: binding (high-salt) – washing – elution (low-salt)
• Adsorption of DNA / RNA in the presence of chaotropic salts
(hydrate shell of DNA / RNA is reversibly removed)
• High-salt / ethanolic washing steps to remove contaminants
• Low-salt or water elution
(hydrate shell is recovered, DNA/RNA is released from the membrane)
|
| Features/Result |
Ready-to-use, sequencing and PCR-grade DNA / RNA
From extra small to extra large scale, from low to high throughput
No alcohol precipitation necessary
Fast and easy procedure
|
|
| NucleoSpin® principle |
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