NucleoBond technology and products

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NucleoBond® Xtra / NucleoBond^® PC technology
and products

Features


	NucleoBond® Xtra	NucleoBond® PC
Technology / separation principle	Anion-exchange chromatography / solid phase extraction
Material / backbone	Modified, macroporous silica gel Very high charge density Very high resolving power Very high binding capacity	Modified, macroporous silica gel High charge density High resolving power High binding capacity
Anion-exchanger group	MAE (methylaminoethanol)
Format	Gravity-flow columns Midi, Maxi	Gravity-flow columns Mini, Midi, Maxi, Mega, Giga, preparative scale
Lysate clarification	NucleoBond® Xtra Column Filters	NucleoBond® Folded Filters
Procedure	Principle: binding (low-pH) – washing – elution (high-pH) – alcohol precipitation • Binding at low pH (interaction between negatively charged DNA backbone and positively charged anion-exchanger material) • Elution at high pH • Alcohol precipitation of eluted plasmid DNA, DNA collection by centrifugation or use of NucleoBond® Finalizer / Finalizer Large
Features / result	Ultra-pure, transfection-grade plasmid DNA The new generation of anion exchangers Xtra fast, Xtra high yields, Xtra convenient	Ultra-pure, transfection-grade plasmid DNA*
	* NucleoBond® technology is also available for isolation of genomic DNA and / or total RNA.

NucleoBond® principle

Products
Plasmid DNA Purification

Transfection-grade plasmid DNA

NucleoBond® Xtra kits	NucleoBond® Xtra Midi
	NucleoBond® Xtra Midi Plus (including NucleoBond® Finalizer)
	NucleoBond® Xtra Maxi
	NucleoBond® Xtra Maxi Plus (including NucleoBond® Finalizer Large)

NucleoBond® PC kits	NucleoBond® PC 20 (Mini)
	NucleoBond® PC 100 (Midi)
	NucleoBond® PC 500 (Maxi)
	NucleoBond® PC 2 000 (Mega)
	NucleoBond® PC 10 000 (Giga)

NucleoBond® kits for large constructs (e.g., BACs, PACs, P1s, cosmids)	NucleoBond® Xtra BAC
	NucleoBond® BAC 100

NucleoBond® AX Columns	NucleoBond® AX 20 (Mini)
	NucleoBond® AX 100 (Midi)
	NucleoBond® AX 500 (Maxi)
	NucleoBond® AX 2 000 (Mega)
	NucleoBond® AX 10 000 (Giga)

NucleoBond® Xtra Buffer Set	NucleoBond® Xtra Buffer Set I (for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs)

NucleoBond® Buffer Set	NucleoBond® Buffer Set I (for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs)

Transfection-grade, endotoxin-free plasmid DNA

NucleoBond® Xtra kits	NucleoBond® Xtra Midi EF
	NucleoBond® Xtra Midi Plus EF (including NucleoBond® Finalizer)
	NucleoBond® Xtra Maxi EF
	NucleoBond® Xtra Maxi EF (including NucleoBond® Finalizer Large)
	NucleoBond® 96 Xtra EF

NucleoBond® PC kits	NucleoBond® PC 500 EF (Maxi)
	NucleoBond® PC 2 000 EF (Mega)
	NucleoBond® PC 10 000 EF (Giga)
	NucleoBond® PC Prep 100 (preparative scale)

RNA / DNA

Total RNA / DNA from cells and tissue

NucleoBond® RNA/DNA kits	NucleoBond® RNA/DNA 80 (Midi)
	NucleoBond® RNA/DNA 400 (Maxi)

Genomic DNA

Genomic DNA from blood, tissue, and bacteria

NucleoBond® CB kits	NucleoBond® CB 100 (Midi)
	NucleoBond® CB 500 (Maxi)

NucleoBond® AXG Columns (to be used in combination with NucleoBond® Buffer Sets)	NucleoBond® AXG 20 (Mini)
	NucleoBond® AXG 100 (Midi)
	NucleoBond® AXG 500 (Maxi)

NucleoBond® Buffer Sets (to be used in combination with NucleoBond® AXG Columns)	NucleoBond® Buffer Set IV (for isolation of genomic DNA from tissue using NucleoBond® AXG Columns)
	NucleoBond® Buffer Set III (for isolation of genomic DNA from bacteria using NucleoBond® AXG Columns)