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NucleoBond® Xtra / NucleoBond® PC technology
and products
Features
NucleoBond® Xtra NucleoBond® PC
Technology /
separation principle
Anion-exchange chromatography / solid phase extraction
Material / backbone Modified, macroporous silica gel
Very high charge density
Very high resolving power
Very high binding capacity
Modified, macroporous silica gel
High charge density
High resolving power
High binding capacity
Anion-exchanger group

MAE (methylaminoethanol)

Format

Gravity-flow columns Midi, Maxi

Gravity-flow columns Mini, Midi, Maxi, Mega, Giga, preparative scale
Lysate clarification NucleoBond® Xtra Column Filters NucleoBond® Folded Filters
Procedure

Principle: binding (low-pH) – washing – elution (high-pH) – alcohol precipitation

• Binding at low pH (interaction between negatively charged DNA backbone and
   positively charged anion-exchanger material)
• Elution at high pH
• Alcohol precipitation of eluted plasmid DNA, DNA collection by centrifugation
   or use of NucleoBond® Finalizer / Finalizer Large

Features / result Ultra-pure, transfection-grade
plasmid DNA
The new generation of anion exchangers
Xtra fast, Xtra high yields, Xtra convenient
Ultra-pure, transfection-grade plasmid DNA*
* NucleoBond® PC technology is also available for isolation of genomic DNA and / or RNA.
NucleoBond® principle

 

  
     
Products
Plasmid DNA Purification
Transfection-grade plasmid DNA
NucleoBond® Xtra kits NucleoBond® Xtra Midi
NucleoBond® Xtra Midi Plus (including NucleoBond® Finalizer)
NucleoBond® Xtra Maxi
NucleoBond® Xtra Maxi Plus (including NucleoBond® Finalizer Large)
NucleoBond® PC kits NucleoBond® PC 20 (Mini)
NucleoBond® PC 100 (Midi)
NucleoBond® PC 500 (Maxi)
NucleoBond® PC 2 000 (Mega)
NucleoBond® PC 10 000 (Giga)
NucleoBond® kits for large constructs
(e.g., BACs, PACs, P1s, cosmids)
NucleoBond® Xtra BAC
NucleoBond® BAC 100
NucleoBond® AX Columns NucleoBond® AX 20 (Mini)
NucleoBond® AX 100 (Midi)
NucleoBond® AX 500 (Maxi)
NucleoBond® AX 2 000 (Mega)
NucleoBond® AX 10 000 (Giga)
NucleoBond® Xtra Buffer Set NucleoBond® Xtra Buffer Set I (for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs)
NucleoBond® Buffer Set NucleoBond® Buffer Set I (for isolation of low-copy plasmids, cosmids, BACs, PACs, and P1 constructs)
Transfection-grade, endotoxin-free plasmid DNA
NucleoBond® Xtra kits
NucleoBond® Xtra Midi EF
NucleoBond® Xtra Midi Plus EF (including NucleoBond® Finalizer)
NucleoBond® Xtra Maxi EF
NucleoBond® Xtra Maxi EF (including NucleoBond® Finalizer Large)
NucleoBond® 96 Xtra EF
NucleoBond® PC kits
NucleoBond® PC 500 EF (Maxi)
NucleoBond® PC 2 000 EF (Mega)
NucleoBond® PC 10 000 EF (Giga)
NucleoBond® PC Prep 100 (preparative scale)
RNA / DNA
RNA / DNA from cells and tissue
NucleoBond® RNA/DNA kits NucleoBond® RNA/DNA 80 (Midi)
NucleoBond® RNA/DNA 400 (Maxi)
RNA from soil
NucleoBond® RNA Soil NucleoBond® RNA Soil
DNA
DNA from blood, tissue, and bacteria
NucleoBond® CB kits NucleoBond® CB 100 (Midi)
NucleoBond® CB 500 (Maxi)
NucleoBond® AXG Columns
(to be used in combination
with NucleoBond® Buffer Sets)
NucleoBond® AXG 20 (Mini)
NucleoBond® AXG 100 (Midi)
NucleoBond® AXG 500 (Maxi)

NucleoBond® Buffer Sets
(to be used in combination
with NucleoBond® AXG Columns)

NucleoBond® Buffer Set IV (for isolation of genomic DNA from
tissue using NucleoBond® AXG Columns)
NucleoBond® Buffer Set III (for isolation of genomic DNA from bacteria using NucleoBond® AXG Columns)
  
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