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NucleoSpin®
FFPE RNA

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NucleoSpin®
FFPE RNA

  
     

NucleoSpin® FFPE RNA

RNA from FFPE samples

Paraffin Dissolver: no use of xylene required

Features 

Improved RNA quality from formalin-fixed, paraffin-embedded samples

 

 

• Enhanced RT-PCR performance – RNA is well decrosslinked and less fragmented
• Very easy paraffin removal – Paraffin Dissolver (patent pending) included
• Minimum number of working steps in a streamlined workflow
• Efficient on-column DNA removal, rDNase included
• Higher sensitivity with concentrated RNA – elution volume as little as 5 μL 

  
Technology Silica-membrane technology
Format Mini spin columns-XS design
Sample material

7 sections (10 μm) of 250 mm total area (< 15 mg paraffin*)

RNA yield Depending on amount and quality of the sample
A260/A280

1.9–2.1

Typical RIN (RNA integrity number) Strongly depending on sample quality (2–6)
Elution volume 5–30 µL
Preparation time 70 min/6 preps
Binding capacity 110 µg


* When using the standard protocol with Paraffin Dissolver. Larger quantities of paraffin can be processed when using additional Paraffin Dissolver or the standard protocol with Xylene for deparaffinization.

Applications**


• Rapid isolation of RNA from formalin-fixed, paraffin-embedded samples, e.g., 
   colon carcinoma (unstained), colon carcinoma (hematoxylin stained), colon mucosa, adenocarcinoma
   of colon ransversum, liver (rat / human), liver carcinoma (hematoxylin stained), lymph node, spleen
   (rat / human), pancreas rat / human; diabetic / non-diabetic), placenta
• Isolation of RNA from fresh and archived FFPE samples
• Isolation of RNA from specimen on object slides (stained or unstained)

• Typical downstream application: RT-PCR

** Kits to be used for research purposes only


 

 

 

  
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Ordering information

Product

Preps

Specification REF
NucleoSpin® FFPE RNA

10

NucleoSpin® FFPE Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), Paraffin Dissolver, buffers, Proteinase K, RNase-free rDNase

740969.10

50

as above 740969.50

250

as above 740969.250

For ordering click here

  
     
  
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