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| NucleoSpin® FFPE RNA |
RNA from FFPE samples
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Paraffin Dissolver: no use of xylene required
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| Features |
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| Improved RNA quality from formalin-fixed, paraffin-embedded samples |
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• Enhanced RT-PCR performance – RNA is well decrosslinked and less fragmented
• Very easy paraffin removal – Paraffin Dissolver (patent pending) included
• Minimum number of working steps in a streamlined workflow
• Efficient on-column DNA removal, rDNase included
• Higher sensitivity with concentrated RNA – elution volume as little as 5 μL
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| Technology |
Silica-membrane technology |
| Format |
Mini spin columns-XS design |
| Sample material |
≤ 7 sections (10 μm) of 250 mm2 total area (< 15 mg paraffin*)
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| RNA yield |
Depending on amount and quality of the sample |
| A260/A280 |
1.9–2.1
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| Typical RIN (RNA integrity number) |
Strongly depending on sample quality (2–6) |
| Elution volume |
5–30 µL |
| Preparation time |
70 min/6 preps |
| Binding capacity |
110 µg |
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| * When using the standard protocol with Paraffin Dissolver. Larger quantities of paraffin can be processed when using additional Paraffin Dissolver or the standard protocol with Xylene for deparaffinization. |
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Applications**
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• Rapid isolation of RNA from formalin-fixed, paraffin-embedded samples, e.g.,
colon carcinoma (unstained), colon carcinoma (hematoxylin stained), colon mucosa, adenocarcinoma
of colon ransversum, liver (rat / human), liver carcinoma (hematoxylin stained), lymph node, spleen
(rat / human), pancreas rat / human; diabetic / non-diabetic), placenta
• Isolation of RNA from fresh and archived FFPE samples
• Isolation of RNA from specimen on object slides (stained or unstained)
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| • Typical downstream application: RT-PCR |
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** Kits to be used for research purposes only
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Ordering information
| Product |
Preps
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Specification |
REF |
| NucleoSpin® FFPE RNA |
10
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NucleoSpin® FFPE Columns, Collection Tubes (2 mL), Collection Tubes (1.5 mL), Paraffin Dissolver, buffers, Proteinase K, RNase-free rDNase
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740969.10 |
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50
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as above |
740969.50 |
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250
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as above |
740969.250 |
For ordering click here
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