New products
Isolation of RNA from fresh or frozen blood
		NucleoSpin® RNA Blood • NucleoSpin® RNA Blood Midi • NucleoSpin® 8 RNA Blood • NucleoSpin® 96 RNA Blood – isolation of RNA from whole blood The NucleoSpin® RNA Blood procedure is based on the simple and efficient direct total blood lysis. The common but cumbersome selective erythrocyte lysis at 4 °C is not necessary, thus allowing a complete processing at room temperature. Genomic DNA is efficiently removed by an on-column rDNase digestion. The NucleoSpin® RNA Midi kit allows isolation of RNA from up to 1300 µL whole blood. For a higher throughput of blood samples the kit is available in the 8-well strip (NucleoSpin® 8 RNA Blood) and 96-well plate (NucleoSpin® 96 RNA Blood) format. • Direct total blood lysis: Very simple and convenient procedure    (no selective erythrocyte lysis at 4 °C — complete processing at RT) • Outstanding RNA yield from up to 1300 µL fresh or frozen whole blood • Efficient on-column rDNase digestion for an increased sensitivity in downstream applications • Compatible with common blood collection tubes and anticoagulants    (e.g., EDTA, citrate, and heparin)   Product page NucleoSpin® RNA Blood    Product page NucleoSpin® RNA Blood Midi    Product page NucleoSpin® 8 / 96 RNA Blood
Isolation of large construct plasmid DNA
		NucleoBond® Xtra BAC – efficient isolation of large construct plasmid DNA The NucleoBond® Xtra BAC is the further development of the NucleoBond® BAC 100 kit. The new kit consists of inserted column filters, known from the products of the NucleoBond® Xtra range. The kit is optimized in yield and prep time. Optimal silica with specific pore sizes realizes highest yield for large constructs. Column design of a wider diameter, inserted column filter and an optimal procedure realizes a prep time of 75 min per prep. The new LyseControl helps to identfy aggregated cells in the lysis buffer and visualize by a color change from blue to color less a completed and sufficient lysis/neutralization of the sample. • Optimized column design: one prep in approximately 30 min • Column filter included: parallel lysate clearing and loading onto the column • Optimal silica material: yields up to 150 μg • Now with LyseControl: visualization of a completed lysis/neutralization • Transfection-grade plasmid DNA using approved anion-exchange technology   Product page NucleoBond® Xtra BAC
Post clean-up of large sized DNA solutions
		NucleoSpin® gDNA Clean-up and NucleoSpin® gDNA Clean-up XS– efficient post clean-up and concentration of large sized DNA The NucleoSpin® gDNA Clean-up and NucleoSpin® gDNA Clean-up XS kits are both for an effective purification of genomic DNA extracted by standard “organic” methods, DNA from enzymatic reactions or for buffer exchange. The proven silica membrane technology is an effective alternative to the widely used concentrator columns for cleaning and concentrating DNA samples. The NucleoSpin® gDNA Clean-up XS kit contains the unique XS spin column for low elution volume (≥ 6 µL). The sample material of NucleoSpin® gDNA Clean-up XS is limited to 2 µg DNA. For larger amounts of genomic DNA (15–25 µg DNA), the Mini spin column format is recommended, included in the NucleoSpin® gDNA Clean-up kit. • Effective post clean-up and concentration of large-sized DNA for successful downstream    applications • High recovery rates up to 90 % for DNA fragments 100 bp–50 kbp • Concentrate your DNA sample easily and faster compared to microdialysis filtrations units Product page NucleoSpin® gDNA Clean-up Product page NucleoSpin® gDNA Clean-up XS
PCR clean-up and gel extraction
		NucleoSpin® Gel and PCR Clean-up – new appearance with a new feature The NucleoSpin® Extract II kit was rebranded to NucleoSpin® Gel and PCR Clean-up. The superior two in one kit with a new feature: binding buffer with pH indicator. Binding column and buffer chemistry remain the same. The new pH indicator in the binding buffer is displaying you the correct pH for optimal kit performance. For further details please visit the product page. • Two applications in one kit, one binding buffer for both applications • Optimal recovery for small fragments down to 50 bp • Binding buffer with pH indicator – working under the right conditions Product page NucleoSpin® Gel and PCR Clean-up
Isolation of small RNA and DNA from plasma and serum
		NucleoSpin® miRNA Plasma – efficient isolation of small RNA from plasma and serum The NucleoSpin® miRNA Plasma procedure is a simple and fast method to isolate small RNA > 15 nt, such as micro RNA (miRNA), from serum or plasma. The DNA can be isolated as well or digested on column to eliminate interference of miRNA genes with downstream applications. • Efficient isolation of small RNA from plasma or serum samples • Superior recovery of small RNA > 15 nt • Optional on-column DNA digest for increased sensitivity in downstream applications Product page NucleoSpin® miRNA Plasma
Viral RNA and DNA from plasma or serum
		NucleoSpin® Dx Virus – CE-IVD marked kit for isolation of viral RNA and DNA from human plasma or serum NucleoSpin® Dx Virusis a mini spin kit for the isolation of viral RNA and DNA from 150 µL human plasma or serum. The kit is CE-IVD marked in accordance to the directive 98/79/EC of the European Union, guaranteeing highest standards of production and quality assurance. NucleoSpin® Dx Virus fits into in-vitro diagnostic workflows*. It is suitable for fresh and frozen samples and compatible with common collection systems, including EDTA, citrate. Features: • CE-marked in accordance with EU Directive 98/79/EC • Fits into in-vitro diagnostic workflows • Compatible with fresh or frozen samples treated with EDTA or citrate • Highly consistent viral RNA and DNA isolation *CE-IVD marked kit in compliance with EU directive98/79/EC. Only available as IVD in certain countries. All other countries research use only! Please inquire. Product page NucleoSpin® Dx Virus
Genomic DNA from FFPE samples
		NucleoSpin® FFPE DNA – easy handling and superior DNA quality The NucleoSpin® FFPE DNA kit provides a convenient, reliable, and fast method to isolate DNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimen. Features: • Easy method for paraffin removal • Superior cleavage of DNA crosslinks • Concentrated DNA: elution in only 5 µL • Integrity preserved DNA for better performance in PCR The procedure omits the use of flammable and malodorous xylene or d-limonene commonly used for deparaffinization and employs the odorless Paraffin Dissolver (patent pending) and allows efficient lysis in a convenient two-phase system. DNA crosslinks, which are a common issue of FFPE tissue, are removed efficiently by incubation with a special designed buffer. The XS-column design of NucleoSpin® FFPE DNA allows elution in as little as 5 µL. Isolated DNA is high in concentration, crosslinks are removed efficiently, and the DNA quality of the sample is well preserved during the complete process (deparaffinization, decrosslink, purification). Thus, isolated DNA shows good performance in all standard downstream applications, such as PCR. Product page NucleoSpin® FFPE DNA
Purification of His-tagged proteins
		Protino® Ni-NTA Agarose and Protino® Ni-NTA Columns (1mL and 5 mL) – save money without making compromises The new product line of the Protino® family for purification of His-tagged proteins features proven market-leading performance for a very competitve price. The Protino® Ni-NTA protduct line includes the ready-to-use bulk resin (25, 100, and 500 mL) and ready-to-use prepacked FPLC™ Columns. Protino® Ni-NTA Agarose has the same technical specifications and performance as the market leading product Ni-NTA Agarose from Qiagen – but with a very attractive price. The Protino® Ni-NTA Columns are packed with crosslinked agarose, which shows higher yields in purifications of large proteins compared to the widely spreaded Superflow matrices. Features: • High capacity and high affinity for any protein size • Up to 50 mg/mL binding capacity • Ready-to-use resin for any application (FPLC™, batch, and gravity-flow) • Purification under denaturing and native conditions • Huge price advantage against market leader Product page Protino® Ni-NTA Agarose Product page Protino® Ni-NTA Columns 1mL/5 mL