Editors
     
  
     
Literature

User manual
NucleoSpin®
RNA XS

Supplementary protocols
NucleoSpin®
RNA XS

Product flyer
NucleoSpin®
RNA XS

  
     
NucleoSpin® RNA XS

RNA from cells and tissue

 

Features
Purification of highly concentrated RNA from smallest samples

• Isolation of RNA from small sample quantities like biopsy material or single cells
• Excellent RNA recovery and integrity
• Concentrated RNA for sensitive downstream applications by elution in as little as 5 μL
• rDNase included for on-column DNA removal
• NucleoSpin® Filters (shredders) – efficient homogenization and reduction of viscosity
• High quality RNA, ready to use for RT-PCR and other applications
• Reducing agent TCEP – no ß-mercaptoethanol necessary
Technology Silica-membrane technology
Format Mini spin column – XS design
Sample material

1-105 cultured cells, < 5 mg human / animal tissue

Fragment size > 200 nt
Typical yield 0.1–1.5 ng (102 HeLa cells)
10–15 ng (103 HeLa cells) 
100–150 ng (104 HeLa cells)
1000–1500 ng (105 HeLa cells)
A260/A280 1.9–2.1
Typical RIN (RNA integrity number) > 9 (depending on sample quality)
Elution volume 5–30 µL
Preparation time 35 min/6 preps
Binding capacity 110 µg
Applications*
• RNA isolation from cultured cells
• RNA isolation from tissue
• RNA isolation from cryosections
• RNA isolation from laser captured cells
• RNA isolation from small amounts of plant material
• RNA isolation from samples stored in RNAlater®
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
   RNase protection assays
* Kits to be used for research purposes only
  
Maximize    

     

Ordering information

Product

Preps

Specification REF
NucleoSpin® RNA XS

10

NucleoSpin® RNA XS Columns with Collection Tubes, NucleoSpin® Filters, Collection Tubes (2 mL), Collection Tubes (1.5 mL),  buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP 740902.10

50

as above 740902.50

250

as above 740902.250

For ordering click here

  
     
  
Copyright 2018 by MACHEREY-NAGEL GmbH & Co. KG   Imprint / Impressum Privacy StatementTerms Of Use