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NucleoBond®
Xtra EF

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NucleoBond® Xtra/Xtra EF

  
     

NucleoBond® Xtra Midi EF / Maxi EF

Endotoxin-free plasmid DNA

Patented endotoxin removal – no incubation on ice

Features
The new generation of anion exchanger – ultra-fast, endotoxin-free plasmid DNA with high yield

• Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells
• Patented endotoxin removal by additional washing step – no extra ice incubation necessary
• Optimized column design  Midi prep in approximately 45 min, Maxi prep in 50 min
• Column filter included  high filter flow-rates, parallel lysate clearing and loading onto the column
• Improved silica material  typical yields of 250 μg (Midi) and 1000 μg (Maxi)
• NucleoBond® Xtra Plus EF kits contain NucleoBond® Finalizers to speed up DNA precipitation


NucleoBond® Xtra Midi EF

NucleoBond® Xtra Maxi EF

Technology

Anion-exchange chromatography

Format

Midi gravity flow columns

Maxi gravity flow columns

Lysate clarification

Column filters

Column filters

Sample material

< 200 mL (high copy)
< 400 mL (low copy)

< 600 mL (high copy)
< 1200 mL (low copy)

Vector size

< 300 kbp

< 300 kbp

Typical yield

400 µg

1000 µg

A260/A280

1.8–1.95

1.8–1.95

Endotoxin level

< 0.05 EU/µg DNA

< 0.05 EU/µg DNA

Isopropanol precipitation

Xtra Midi EF

Centrifugation

Xtra Midi Plus EF

NucleoBond® Finalizer

Xtra Maxi EF

Centrifugation

Xtra Maxi Plus EF

NucleoBond® Finalizer Large

Preparation time

85 min/prep

45 min/prep

90 min/prep

50 min/prep

Applications
• High-copy plasmids from E. coli
• Low-copy plasmids from E. coli (increased buffer volumes required, see "Ordering information")
  
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Ordering information

Product

Preps

Specification REF
NucleoBond® Xtra Midi EF kits


NucleoBond® Xtra Midi EF

10

NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A 740420.10

50

as above 740420.50
NucleoBond® Xtra Midi Plus EF

10

NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizer, buffers, RNase A

740422.10

50

as above 740422.50
NucleoBond® Xtra® Maxi EF kits


NucleoBond® Xtra Maxi EF

10

NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A 740424.10

50

as above 740424.50
NucleoBond® Xtra Maxi Plus EF

10

NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizer Large, buffers, RNase A

740426.10

50

as above 740426.50




Product accessories

Pack of

Specification REF
NucleoBond® Xtra Combi Rack

1

for use with NucleoBond® Xtra Midi, Maxi, AX 100, 500, 2000, and 10000 Columns 740415
NucleoBond® Xtra EF Buffer Set I

1

for isolation of endotoxin-free low-copy plasmids, cosmids, BACs, PACs, and P1 constructs,
only applicable with NucleoBond® Xtra EF kits, sufficient for 10 Xtra EF Maxipreps or 20 Xtra EF Midipreps. Set includes 150 mL of buffers RES-EF, LYS-EF, NEU-EF, and RNase A
740427

For ordering click here

  
     
  
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