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NUCLEOGEL® SUGAR · NUCLEOGEL® ION 300 OA
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polymer-based HPLC phases for separation of sugars
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Features
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sulfonated spherical PS/DVB resins in different ionic forms; mean particle size 10 µm, pore size 100 Å
separation mechanism includes steric exclusion, ligand exchange and partition effects, ligand exchange being the predominant force, since the hydrated metal ions form strong interactions with the hydroxyl groups of the sample molecules. The intensity of these interactions decreases in the sequence Pb, Ca, Na
eluent in column for Ca, Na and Pb phases: water + 0.02 % azide
recommended operating temperatures: 60 – 95 °C; maximum pressure 100 bar
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NUCLEOGEL® ION 300 OA:
H+ form for separation of sugars, alcohols and organic acids ∙ USP L17
eluent in column 0.01 N H2SO4
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NUCLEOGEL® SUGAR Ca:
for separation of mono- and oligosaccharides, sugar alcohols ∙ USP L19
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NUCLEOGEL® SUGAR Na:
for separation of oligosaccharides from starch hydrolysates and food ∙ USP L58
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NUCLEOGEL® SUGAR Pb:
for separation of mono- and disaccharides from food and biological samples ∙ USP L34
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Ordering information
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Length
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300 mm
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Guard columns*
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NUCLEOGEL® ION 300 OA
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| Valco type analytical columns |
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7.8 mm ID
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719501
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719537
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NUCLEOGEL® SUGAR Ca
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| Valco type analytical columns |
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6.5 mm ID
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719531
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719535 |
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| NUCLEOGEL® SUGAR Pb |
| Valco type analytical columns |
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7.8 mm ID
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719530
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719534 |
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| NUCLEOGEL® SUGAR Na |
| Valco type analytical columns |
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7.8 mm ID
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719532
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719536 |
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| Valco type guard columns cartridges are 21 x 4 mm, require guard column holder C (Cat. No. 719538) and are supplied in packs of 2 (all other columns in packs of 1). |
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