Editors

Methylation with DMF-DMA

Method:
Matrix
Application-No.:
GC

213070




Reagent:
N,N-dimethylformamide dimethylacetal (DMF-DMA)






REF numbers:
701430.201; 701430.110



Procedure:
Add 1 mL of a mixture of DMF-DMA and pyridine (1:1)
to 1–50 mg analyt (e.g., fatty acids). As soon as a clear solution has
formed, the sample can be injected. However, it is recommended
to heat the solution to 60–100 °C for 10–15 minutes.



Conditions:
Methylation with DMF-DMA can be applied for fatty acids,
primary amines and (partially) amino acids forming
N-dimethyl-aminomethylene amino acid methyl esters
[Thenot et al., Anal. Letters 5 (1972) 217–223, 519–529]. DMF-DMA
is a poor solvent, for this reason it is necessary to
use a mixture of DMF-DMA with pyridine, THF, acetone
(barbiturates) or another solvent.



Subsequent analysis:
GC



Author(-s):
MACHEREY-NAGEL
Source:
GC department (2007)



Keywords:
derivatization; methylation; N,N-dimethylformamide dimethylacetal; DMF-DMA; fatty acids; amines; primary amines; amino acids; FAMEs




















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