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NucleoSpin
RNA XS

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NucleoSpin
RNA XS

  
     

NucleoSpin® RNA XS

Total RNA from cells and tissue

5 µl elution volume ® highly concentrated RNA

Features 
Purification of highly concentrated RNA from smallest samples

• Isolation of RNA from low amount of sample material like biopsy material
   or single cells
• Elution in only 5 µl resulting in concentrated RNA for sensitive downstream
   applications
• rDNase included for optional on-column DNA removal
• NucleoSpin Filters (Shredders) included ® efficient homogenization and reduction of viscosity
• High quality RNA, ready-to-use for RT-PCR and other applications

  
Technology Silica-membrane technology
Format XS spin columns
Sample material

Small amounts of tissue < 5 mg
1-100000 cultured cells

Fragment size > 200 b
Typical yield

102 HeLa cells: 0.1-1.5 ng
103 HeLa cells: 10-15 ng
104 HeLa cells: 100-150 ng
105 HeLa cells: 1000-1500 ng

A260/280 1.9-2.1
Typical RIN (RNA integrity number) > 9 (depending on sample quality)
Elution volume 5-20 µl
Preparation time 40 min/12 preps
Binding capacity 90 µg

Applications*

• Total RNA isolation from cultured cells
• Total RNA isolation from tissue
• Total RNA isolation from cryosections
• Total RNA isolation from laser captured cells
• Total RNA isolation from small amounts of plant material
• Total RNA isolation from samples stored in
RNAlater®

RNA suitable for e.g. real-time RT-PCR, Northern blotting, primer extension, array technology, RNase
   protection assays

* Kits to be used for research purposes only

  
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Ordering information

Product

Preps

Specification Reference
NucleoSpin RNA XS

10

NucleoSpin RNA XS Columns, Collection Tubes (2 ml), Collection Tubes (1.5 ml), NucleoSpin Filters, buffers, RNase-free rDNase, Carrier RNA, Reducing Agent TCEP 740902.10

50

as above 740902.50

250

as above 740902.250

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