NucleoBond® Xtra Midi EF/Maxi EF	Endotoxin-free plasmid DNA
68% time-saving, 100% increased yields
Features
The new generation of anion exchangers - ultra-fast plasmid preps with high yields of endotoxin-free plasmid DNA
• Save up to 68% of your time, Midi prep less than 45 min, Maxi prep less than 48 min • Increase your yield of endotoxin-free plasmid DNA up to 100%, typical yield Midi 250 µg,        Maxi 1000 µg • DNA with less than 0.05 EU/µg DNA suitable for transfection of highly sensitive cells • Efficient removal of endotoxins by just one additional washing step compared to standard    procedure of NucleoBond Xtra ® highest convenience • Column filter included for lysate clarification: high filter flow rates, parallel clearing of lysates and    loading onto the column ® time saving • NucleoBond Xtra columns:     large diameter + low silica resin bed = high column flow rates ® time saving • NucleoBond Xtra Plus EF kits contain NucleoBond Finalizers to speed up DNA precipitation    ® time saving • Improved silica material with higher DNA binding capacity ® higher yields • Optimized EF buffers ® highest yields and most efficient endotoxin removal

	NucleoBond Xtra Midi EF		NucleoBond Xtra Maxi EF
Technology	Anion-exchange chromatography
Format	Midi gravity flow columns		Maxi gravity flow columns
Lysate clarification	Column filters (included)		Column filters (included)
Sample material	< 200 ml (high copy) < 400 ml (low copy)		< 600 ml (high copy) < 1200 ml (low copy)
Vector size	< 300 kbp		< 300 kbp
Typical yield	> 250 µg		> 1000 µg
A260/280	1.8-1.95		1.8-1.95
Endotoxin level	< 0.05 EU/µg DNA		< 0.05 EU/µg DNA
Isopropanol precipitation	Xtra Midi EF Centrifugation	Xtra Midi Plus EF NucleoBond Finalizer	Xtra Maxi EF Centrifugation	Xtra Maxi Plus EF NucleoBond Finalizer Large
Preparation time	100 min/4 preps	45 min/4 preps	103 min/4 preps	48 min/4 preps
Applications
• High-copy plasmids from E. coli • Low-copy plasmids from E. coli*
* Increased buffer volumes required, see "Ordering information"