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NucleoSpin® gDNA Clean-up XS

  
     
NucleoSpin® gDNA Clean-up XS

 

Features 
Post clean-up and concentration of small amounts of large DNA fragments

• Efficient post clean-up of large DNA fragments for successful downstream applications
• Recommended for small DNA quantities extracted from, e.g., forensic samples
• Unique XS spin column for low elution volume (> 6 µL) – highly concentrated DNA
• Easier and faster than microdialysis filtration units
• 20 min/6 preps – time-saving procedure

 

Technology Silica-membrane technology
Format Mini spin columns – XS design
Sample material

< 400 µL aqueous DNA solution (< 2 µg DNA)

Fragment size 100 bp–approx. 50 kbp
Typical recovery 60–70 %
A260/A280 1.8–1.9
Elution volume 6–10 µL
Preparation time 20 min/6 preps
Binding capacity 3 µg
Applications
• Clean-up and concentration of pre-purified DNA (organic extractions)
• Clean-up and concentration of DNA from enzymatic reactions
Not recommended for gel or PCR clean-up! For this application, please use NucleoSpin® Gel and PCR Clean-up.
• Typical downstream applications: PCR, enzymatic reactions, Southern blotting, qPCR, STR amplification
  
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References

Forensic Sci Int Genet. 2011 Jun;5(3):226-30. Epub 2010 Apr 15.
The NucleoSpin® DNA Clean-up XS kit for the concentration and purification of genomic DNA extracts: An alternative to microdialysis filtration.
Hudlow WR, Krieger R, Meusel M, Sehhat JC, Timken MD, Buoncristiani MR.
California Department of Justice Jan Bashinski DNA Laboratory, 1001 W. Cutting Blvd., Suite 110, Richmond, CA 94804, USA.

Note: NucleoSpin® DNA Clean-up XS has been renamed and is now available as NucleoSpin® gDNA Clean-up XS
View ABSTRACT (Reference I)

Forensic Sci Int Genet. 2013 Jan;7(1):209-13. Epub 2012 Jul 10.
PCR inhibitor removal using the NucleoSpin® DNA Clean-up XS kit 
Faber KL, Person EC, Hudlow WR.
California Department of Justice Jan Bashinski DNA Laboratory, 1001 W. Cutting Blvd., Suite 110, Richmond, CA 94804, USA.

Note: NucleoSpin® DNA Clean-up XS has been renamed and is now available as NucleoSpin® gDNA Clean-up XS
View ABSTRACT (Reference II)
  
     

Ordering information

Product

Preps

Specification REF
NucleoSpin® gDNA Clean-up XS

10

NucleoSpin® gDNA Clean-up XS Columns,
Collection Tubes (2 mL), buffers
740904.10

50

as above 740904.50

250

as above 740904.250

For ordering click here

  
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