| Recommended accessories for use of the NucleoSpin® 96 Tissue Core Kits |
| The NucleoSpin 96 Tissue Core kit provides the buffers, Proteinase K and NucleoSpin Tissue Binding Plate only. Accessory plates (e.g. lysis plates, elution plates) are not provided with the core kit. The user can individually select additional consumables from a variety of suitable accessory plates according to his requirements for highest flexibility. |
| Protocol step |
Suitable consumables not supplied with the Core Kits
|
Remarks |
| Lysis |
4 x Round-well Block with 12 Cap Strips
per 4 x 96 preps |
If residual hair and/or bones in the lysate must be removed by centrifugation and transfer of the supernatant an additional Round-well Block per 96 preps is necessary. |
|
or |
|
4 x Rack with Tube Strips, with
12 Cap Strips per 4 x 96 preps |
|
Adjustment of binding
conditions |
48 Cap Strips per 4 x 96 preps |
When using Round-well Block or MN Tube Strips for lysis, new Cap Strips are required for closure of wells after adding Buffer BQ1 and ethanol |
|
or |
|
|
4 x MN Square-well Block
per 4 x 96 preps |
Recommended for automated processing only |
| Binding of DNA to the membrane |
4 x MN Wash Plate per 4 x 96 preps
|
MN Wash Plate minimizes the risk of cross-contamination (vacuum processing only) |
|
2 x MN Square-well Block |
For waste collection during centrifugation (reusable) |
| Elution |
4 x Rack with Tube Strips, with
Cap Strips per 4 x 96 preps
|
Round-well Blocks and Tube Strips can be closed with Cap Strips |
|
or |
|
4 x Round-well Block with 12 Cap Strips
per 4 x 96 preps |