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Protino® Glutathione Agarose 4B
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Purification of GST-tag proteins
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leading performance, unbeatable price
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Features
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| Protino Glutathione Agarose 4B, the cost-effective alternative for GST-tag protein purification |
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 • Highest performance equivalent to Glutathione Sepharose™ 4B ® Converting is simple
• Simply copy and paste your procedure, no optimization or protocol change necessary
• For batch binding, gravity flow, and FPLC™ ® highest flexibility of applications
• Suitable for small proteins, large protein complexes, proteins with low expression rates
® universal use
• Huge price advantage against market leader ® great performance for an unbeatable price
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| Technology |
Affinity chromatography
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| Ligand |
Glutathione, linked via sulfur atom |
| Spacer arm |
12 atoms
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| Format |
75% (v/v) aqueous suspension containing 20% ethanol
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Matrix
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4% beaded agarose
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| Bead size |
90 µm |
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Binding capacity*
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>8 mg recombinant GST/ml medium
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| Recommended flow rates |
| 1 ml bed volume (column with 6.6 mm inner diameter): |
| Sample loading**: |
0.2 - 1.0 ml/min |
| Washing/elution: |
1.0 ml/min |
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| 10 ml bed volume (column with 16 mm inner diameter): |
| Sample loading**: |
0.5 - 5.0 ml/min |
| Washing/elution: |
5.0 ml/min |
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| Max. linear flow rate |
250 cm/h |
| Chemical stability |
Protino Glutathione Agarose 4B withstands incubation in 0.1 M acetate pH 4,
0.1 M NaOH, 70% ethanol, or 6 M guanidine hydrochloride without significant loss of protein yield.
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| Storage temperature |
4-8°C |
| Note |
Do not autoclave the gel |
* Binding capacity will vary for each GST-tagged protein
** Slow flow rates are recommended for the loading step to allow maximal binding of the GST-tagged protein |
| Applications |
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• Purification of GST-tagged proteins
• Batch binding, gravity-flow column chromatography, MPLC/FPLC™ applications |
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Ordering information
| Product |
Pack of
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Specification |
Reference |
| Protino Glutathione Agarose 4B |
10 ml
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10 ml settled beaded agarose |
745500.10 |
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100 ml
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100 ml settled beaded agarose |
745500.100 |
For ordering click here
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