|
RNA from cells and tissue
|
|
Format |
Sample
|
Product
|
|
Single prep (silica-membrane technology)
|
|
|
|
|
|
Mini spin columns |
< 30 mg tissue,
< 5 x 106 cultured cells
|
NucleoSpin® RNA II |
|
|
|
|
XS spin columns |
< 5 mg tissue,
1–105 cultured cells
|
NucleoSpin® RNA XS |
|
|
|
|
|
|
Midi spin columns |
< 200 mg tissue,
< 5 x 107 cultured cells
|
NucleoSpin® RNA L |
|
|
Gravity-flow columns
(anion-exchange chromatography) |
< 100 mg tissue,
< 5 x 107 cultured cells
|
NucleoBond® RNA/DNA |
|
Manual and automated high throughput (HTP) |
|
|
|
|
|
|
8-well strips
(silica-membrane technology) |
< 30 mg tissue,
< 107 cultured cells
|
NucleoSpin® 8 RNA
|
| NucleoSpin® 8 RNA Core Kit |
|
|
|
|
|
|
|
96-well plates
(silica-membrane technology) |
< 30 mg tissue,
< 107 cultured cells
|
NucleoSpin® 96 RNA |
| NucleoSpin® 96 RNA Core Kit |
|
|
|
|
|
|
96-well system
(magnetic-bead technology) |
< 20 mg tissue,
< 2 x 106 cultured cells
|
NucleoMag® 96 RNA |
|
|
MicroRNA
|
|
|
Format |
Sample
|
Product
|
|
Single prep (silica-membrane technology)
|
|
|
|
|
|
|
Mini spin columns |
< 30 mg human / animal tissue,
< 50 mg plant tissue,
< 107 cultured cells,
< 150 μL reaction mixture
|
NucleoSpin® miRNA |
|
|
|
|
|
|
Mini spin columns |
300 µL blood plasma or serum (up to 900 µLwith multiple loading)
|
NucleoSpin® miRNA Plasma |
|
|
RNA, DNA, and protein
|
|
Format |
Sample
|
Product |
|
|
Single prep (silica-membrane technology)
|
|
|
|
Total RNA, DNA and protein isolation |
|
|
|
Mini spin columns |
< 30 mg tissue,
< 100 mg plant tissue,
< 5 x 106 cultured cells
|
NucleoSpin® TriPrep |
|
|
|
Total RNA and protein isolation |
|
Mini spin columns |
< 30 mg tissue,
< 100 mg plant tissue,
< 5 x 106 cultured cells
|
NucleoSpin® RNA/Protein |
|
|
|
Total RNA and DNA isolation |
|
Buffer set |
< 30 mg tissue,
< 100 mg plant tissue,
< 5 x 106 cultured cells
|
NucleoSpin® RNA/DNA Buffer Set |
|
|
|
Protein quantification |
|
Buffer and reagent set |
|
Protein Quantification Assay |
|
|
|
|
|
|
RNA from blood
|
|
Format |
Sample
|
Product |
|
|
Single prep (silica-membrane technology)
|
|
|
|
| Mini spin columns |
< 400 µL whole blood
|
NucleoSpin® RNA Blood |
|
|
|
|
|
|
Mini spin columns |
< 1300 µL whole blood
|
NucleoSpin® RNA Blood Midi |
|
Manual and automated high throughput (HTP) |
|
|
|
|
|
|
8-well strips
(silica-membrane technology) |
< 400 µL whole blood
|
NucleoSpin® 8 RNA Blood |
|
|
|
|
|
|
96-well plates
(silica-membrane technology) |
< 400 µL whole blood
|
NucleoSpin® 96 RNA Blood |
|
|
|
|
|
RNA from plant
|
|
Format |
Sample
|
Product |
|
|
Single prep (silica-membrane technology)
|
|
|
|
| Mini spin columns |
< 100 mg plant tissue
|
NucleoSpin® RNA Plant |
|
|
RNA from FFPE samples
|
|
Format |
Sample
|
Product |
|
Single prep (silica-membrane technology)
|
|
|
Mini spin columns -
XS design |
≤ 7 sections (10 μm) of 250 mm2
total area (< 15 mg paraffin)
|
NucleoSpin® FFPE RNA |
|
|
|
|
|
|
Mini spin columns -
XS design
|
≤ 7 sections (10 μm) of 250 mm2
total area (< 15 mg paraffin)
|
NucleoSpin® FFPE RNA/DNA |
|
|
RNA clean-up
|
|
Format |
Sample
|
Product |
|
|
Single prep (silica-membrane technology)
|
|
|
|
| Mini spin columns |
≤ 200 μL phenol / chloroform extract
or reaction mixture
|
NucleoSpin® RNA Clean-up |
|
|
|
|
XS spin columns |
≤ 300 μL phenol / chloroform extract
or reaction mixture
|
NucleoSpin® RNA Clean-up XS |
|
| Poly(A) mRNA from total RNA |
|
Format |
Sample
|
Product |
|
Single prep (affinity chromatography) |
|
|
|
|
|
|
Latex beads |
250–1000 μg total RNA
|
NucleoTrap® mRNA |