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User manuals RNA 

Overview flyer
MN guide to RNA purification 

  
     

NEWS

RNA/DNA from saliva samples

Partner Program
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RNA isolation and clean-up
 

RNA from cells and tissue


Format

Sample 

Product

Single prep (silica-membrane technology)




Mini spin columns

< 30 mg tissue,
< 5 x 106 cultured cells

NucleoSpin® RNA II




XS spin columns

< 5 mg tissue,
1–105 cultured cells

NucleoSpin® RNA XS
Midi spin columns

< 200 mg tissue,
< 5 x 107 cultured cells

NucleoSpin® RNA L
Gravity-flow columns
(anion-exchange chromatography)

< 100 mg tissue,
< 5 x 107 cultured cells

NucleoBond® RNA/DNA

Manual and automated high throughput (HTP) 

8-well strips
(silica-membrane technology)

< 30 mg tissue,
< 107 cultured cells

NucleoSpin® 8 RNA

NucleoSpin® 8 RNA Core Kit




96-well plates
(silica-membrane technology)

< 30 mg tissue,
< 107 cultured cells

NucleoSpin® 96 RNA
NucleoSpin® 96 RNA Core Kit





96-well system
(magnetic-bead technology)

< 20 mg tissue,
< 2 x 106 cultured cells

NucleoMag® 96 RNA

MicroRNA

 

Format

Sample

Product


Single prep (silica-membrane technology)





Mini spin columns

< 30 mg human / animal tissue,
< 50 mg plant tissue,
< 107 cultured cells,
< 150 μL reaction mixture

NucleoSpin® miRNA

Mini spin columns

300 µL blood plasma or serum (up to 900 µLwith multiple loading) 

NucleoSpin® miRNA Plasma

RNA, DNA, and protein


Format

Sample

Product
 

Single prep (silica-membrane technology)




Total RNA, DNA and protein isolation
Mini spin columns

< 30 mg tissue,
< 100 mg plant tissue,
< 5 x 106 cultured cells

NucleoSpin® TriPrep



Total RNA and protein isolation

Mini spin columns

< 30 mg tissue,
< 100 mg plant tissue,
< 5 x 106 cultured cells

NucleoSpin® RNA/Protein



Total RNA and DNA isolation

Buffer set

< 30 mg tissue,
< 100 mg plant tissue,
< 5 x 106 cultured cells

NucleoSpin® RNA/DNA Buffer Set



Protein quantification

Buffer and reagent set
Protein Quantification Assay




RNA from blood

Format

Sample

Product

Single prep (silica-membrane technology)

Mini spin columns

< 400 µL whole blood

NucleoSpin® RNA Blood

Mini spin columns

< 1300 µL whole blood

NucleoSpin® RNA Blood Midi
Manual and automated high throughput (HTP) 

8-well strips
(silica-membrane technology)

< 400 µL whole blood

NucleoSpin® 8 RNA Blood

96-well plates
(silica-membrane technology)

< 400 µL whole blood

NucleoSpin® 96 RNA Blood

RNA from plant


Format

Sample

Product

Single prep (silica-membrane technology)




Mini spin columns

< 100 mg plant tissue

NucleoSpin® RNA Plant

RNA from FFPE samples


Format

Sample

Product

Single prep (silica-membrane technology)

 

Mini spin columns - 
XS design

≤ 7 sections (10 μm) of 250 mm2
total area (< 15 mg paraffin)

NucleoSpin® FFPE RNA




 

Mini spin columns - 
XS design

≤ 7 sections (10 μm) of 250 mm2
total area (< 15 mg paraffin)

      NucleoSpin® FFPE RNA/DNA

RNA clean-up


Format

Sample

Product

Single prep (silica-membrane technology)




Mini spin columns

≤ 200 μL phenol / chloroform extract
or reaction mixture

NucleoSpin® RNA Clean-up

 



XS spin columns

≤ 300 μL phenol / chloroform extract
or reaction mixture

NucleoSpin® RNA Clean-up XS

Poly(A) mRNA from total RNA

Format

Sample

Product

Single prep (affinity chromatography)




 

Latex beads

250–1000 μg total RNA 

NucleoTrap® mRNA

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 
  
     
  
   
  
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