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User manuals RNA

Supplementary protocols
RNA

Overview flyer
RNA purification guide

Sample homogenization
NucleoSpin® Bead Tubes 

  
     
INFO

RNA/DNA from saliva samples

Partner Program
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RNA
RNA from cells and tissue
  
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Format

Sample 

Product

Single prep (silica-membrane technology)




Mini spin columns  < 30 mg tissue,
< 1 x 107 cultured cells
NucleoSpin® RNA Plus
XS spin columns

< 5 mg tissue,
1–105 cultured cells

NucleoSpin® RNA Plus XS
Mini spin columns

< 30 mg tissue,
< 1 x 106 cultured cells

NucleoSpin® RNA
XS spin columns < 5 mg tissue,
1–105 cultured cells
NucleoSpin® RNA XS

 

Reagent < 100 mg tissue,
< 1 x 107 cultured cells,
< 0.4 mL (viral) fluids
NucleoZOL
Column and buffer set < 500 µL NucleoZOL
sample
NucleoSpin® RNA Set for NucleoZOL
Midi spin columns

< 200 mg tissue,
< 5 x 107 cultured cells

NucleoSpin® RNA Midi
Gravity-flow columns
(anion-exchange chromatography)

< 100 mg tissue,
< 2 x 107 cultured cells

NucleoBond® RNA/DNA
Manual and automated high throughput (HTP) 
8-well strips
(silica-membrane technology)

< 20 mg tissue,
< 107 cultured cells

NucleoSpin® 8 RNA
     NucleoSpin® 8 RNA Core Kit
96-well plates
(silica-membrane technology)

< 20 mg tissue,
< 107 cultured cells

NucleoSpin® 96 RNA
     NucleoSpin® 96 RNA Core Kit
Flexible
(magnetic-bead  technology)

< 20 mg tissue,
< 2 x 106 cultured cells

NucleoMag® RNA

     

MicroRNA

  
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Format

Sample

Product


Single prep (silica-membrane technology)





Mini spin columns

< 107 cultured cells,
< 30 mg tissue,
< 50 mg plant tissue,
< 150 μL reaction mixture

NucleoSpin® miRNA

Mini spin columns

< 300 µL plasma, serum
(< 900 µLwith multiple loading steps) 

NucleoSpin® miRNA Plasma
Precpitation
Solution serum, plasma

Exosome Precipitation

Solution (Serum/Plasma)  
Solution urine Exosome Precipitation
Solution (Urine)

     

RNA, DNA, and protein

  
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RNA from blood

  
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Total RNA from FFPE samples

  
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RNA from plant

  
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RNA from soil

  
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Format Sample Product

        

Single prep (silica-membrane technology)

 

Midi gravity flow columns < 2 g of soil
NucleoBond® RNA Soil





     

RNA from stool

  
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Format Sample Product

        

Single prep (silica-membrane technology)

 

Mini spin columns

~ 200 mg fresh or frozen stool samples 

NucleoSpin® RNA Stool





     
Poly(A) mRNA from total RNA
  
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RNA clean-up

  
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