NucleoSpin® Soil	Isolation of genomic DNA from soil and sediments
high yield and purity from diverse samples
Features
Isolation of total DNA from diverse soil types
• Alternative lysis systems ... high flexibility!    Highest yield from diverse samples    Two different lysis buffers and an optional additive allow to tune for the best result    Designed for Gram-positive bacteria, archaea, fungi, and algae in soil, sludge, or sediment • Most efficient cell disruption ... detection of difficult to lyse microorganisms!    Ready-to-use NucleoSpin Bead Tubes with ceramic beads included • Complete removal of PCR inhibitors ... ready to use for PCR -> no dilution of rare targets!    NucleoSpin Inhibitor Removal Columns and efficient wash buffers eliminate PCR inhibitors     DNA can mostly be used undiluted as PCR template

  

Technology	Silica-membrane technology
Format	Mini spin columns
Sample material	< 500 mg soil, sludge, or sediment
Typical yield	2 – 10 µg
Elution volume	30 – 100 µl
Preparation time	< 90 min (10 preps)
Binding capacity	50 µg
Applications*
• Total DNA from microorganisms in soil and sediment • Free DNA in soil                                                                                                       • Quantify and detect microorganisms from environmental samples • DNA suitable for Southern blotting, array technology, etc.
*  Kits to be used for research purposes only