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Instruction leaflet:
NUCLEODUR®
RP columns


  
   
NUCLEODUR® Sphinx RP
High purity silica phase for HPLC
Bifunctional RP phase, balanced ratio of propylphenyl and C18 ligands
endcapped · 15 % C · USP L1 / L11
Properties
Key features:
Distinct selectivity based on well-balanced bifunctional surface coverage
Widens the scope for method development based on additional π-π interactions
Suitable for LC/MS due to low bleeding characteristics
Technical characteristics:
Octadecyl and propylphenyl modified silica
pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm
carbon content 15 %; pH stability 1–10;
high reproducibility and consistent quality
Recommended application:
Quinolone antibiotics, sulfonamides, xanthines, substituted aromatics
For applications click here.


Standard program* of columns packed with NUCLEODUR® Sphinx RP
Column system Particle size: 1.8 µm 3 µm 5 µm
Analytical EC columns (standard system, 2–4.6 mm)
  Microbore columns (MB, 1 mm)  
Preparative VarioPrep columns (VP, ≥ 8 mm)    
if you do not know our HPLC column systems, click the "i" for further information
* Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request.

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EC standard columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water
Length -> 30 mm 50 mm 75 mm 100 mm 125 mm 150 mm 250 mm
NUCLEODUR® Sphinx RP, 1.8 µm
2 mm ID 760821.20 760822.20 760825.20 760823.20   760824.20  
3 mm ID 760821.30 760822.30   760823.30      
4 mm ID 760821.40 760822.40   760823.40      
4.6 mm ID 760821.46 760822.46   760823.46      
EC guard columns*: 4 x 2 mm 761920.20   4 x 3 mm 761920.30  
NUCLEODUR® Sphinx RP, 3 µm
2 mm ID   760806.20   760812.20 760807.20 760805.20 760808.20
3 mm ID   760806.30   760812.30 760807.30 760805.30 760808.30
4 mm ID   760806.40   760812.40 760807.40 760805.40 760808.40
4.6 mm ID   760806.46 760813.46 760812.46 760807.46 760805.46 760808.46
EC guard columns*: 4 x 2 mm 761921.20   4 x 3 mm 761921.30  
CC guard columns**: 8 x 3 mm 761557.30   8 x 4 mm 761557.40  
NUCLEODUR® Sphinx RP, 5 µm
2 mm ID   760800.20   760809.20 760801.20 760802.20 760803.20
3 mm ID   760800.30   760809.30 760801.30 760802.30 760803.30
4 mm ID   760800.40   760809.40 760801.40 760802.40 760803.40
4.6 mm ID   760800.46 760815.46 760809.46 760801.46 760802.46 760803.46
EC guard columns*: 4 x 2 mm 761922.20   4 x 3 mm 761922.30  
CC guard columns**: 8 x 3 mm 761550.30   8 x 4 mm 761550.40  
Guard columns in packs of 3, EC columns in packs of 1

Guard columns for EC columns   EC columns with ID Use guard
column holder
      2 mm 3 mm 4 mm 4.6 mm
* Column Protection System EC guard column 4/2 4/3 4/3 4/3 REF 718966
** ChromCart® guard column system CC guard column 8/3 8/3 8/4 8/4 REF 721359
Custom-packed EC columns with other dimensions are available on request.
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Microbore columns for analytical HPLC
Ordering information
Eluent in columns acetonitrile – water
Length -> 50 mm 100 mm 125 mm 150 mm 250 mm
NUCLEODUR® Sphinx RP, 3 µm
1 mm ID 717686.10 717685.10   717687.10  
NUCLEODUR® Sphinx RP, 5 µm
1 mm ID 717680.10 717681.10 717682.10 717683.10 717684.10
All columns in packs of 1. Guard columns for microbore columns on request!
On request, Microbore columns are also available in lengths of 40, 60, 200, and 300 mm and with 0.05 · 0.075 · 0.1 · 0.15 · 0.3 · 0.4 · 0.5 · 0.75 and 1.5 mm ID.
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VarioPrep columns for preparative HPLC
Ordering information
Eluent in column acetonitrile – water
Length 10 mm ID 21 mm ID 32 mm ID 40 mm ID
NUCLEODUR® Sphinx RP, 5 µm
50 mm 762372.100 762372.210    
125 mm 762375.100 762375.210    
150 mm       762371.400
250 mm 762373.100 762373.210 762373.320 762373.400
VP guard columns with NUCLEODUR® Sphinx RP
  8 mm ID 16 mm ID 32 mm ID
10 mm* 762390.80 762390.160    
15 mm*     762392.320
* VP guard column Size Pack of for VP column required holder REF
VP 10/8 10 x 8 mm ID 2 10 mm ID 8 mm 718251
VP 10/16 10 x 16 mm ID 2 21 mm ID 16 mm 718256
VP 15/32 15 x 32 mm ID 1 32–40 mm ID 32 mm 718253
Custom-packed VP columns with other dimensions are available on request.
For details of the VarioPrep column system click here.
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