NUCLEODUR Sphinx RP

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Instruction leaflet:
NUCLEODUR®
RP columns

NUCLEODUR® Sphinx RP
High purity silica phase for HPLC
Bifunctional RP phase, balanced ratio of propylphenyl and C18 ligands endcapped · 15 % C · USP L1 / L11
Properties

	Key features: Distinct selectivity based on well-balanced bifunctional surface coverage Widens the scope for method development based on additional π-π interactions Suitable for LC/MS due to low bleeding characteristics
	Technical characteristics: Octadecyl and propylphenyl modified silica pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm carbon content 15 %; pH stability 1–10; high reproducibility and consistent quality
	Recommended application: Quinolone antibiotics, sulfonamides, xanthines, substituted aromatics For applications click here.

Standard program* of columns packed with NUCLEODUR® Sphinx RP
Column system		Particle size:	1.8 µm	3 µm	5 µm
Analytical	EC columns (standard system, 2–4.6 mm)
	Microbore columns (MB, 1 mm)
Preparative	VarioPrep columns (VP, ≥ 8 mm)
if you do not know our HPLC column systems, click the "i" for further information
* Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request.

EC standard columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water

Length ->	30 mm	50 mm	75 mm	100 mm	125 mm	150 mm	250 mm
NUCLEODUR® Sphinx RP, 1.8 µm
2 mm ID	760821.20	760822.20	760825.20	760823.20		760824.20
3 mm ID	760821.30	760822.30		760823.30
4 mm ID	760821.40	760822.40		760823.40
4.6 mm ID	760821.46	760822.46		760823.46
EC guard columns*:		4 x 2 mm	761920.20		4 x 3 mm	761920.30
NUCLEODUR® Sphinx RP, 3 µm
2 mm ID		760806.20		760812.20	760807.20	760805.20	760808.20
3 mm ID		760806.30		760812.30	760807.30	760805.30	760808.30
4 mm ID		760806.40		760812.40	760807.40	760805.40	760808.40
4.6 mm ID		760806.46	760813.46	760812.46	760807.46	760805.46	760808.46
EC guard columns*:		4 x 2 mm	761921.20		4 x 3 mm	761921.30
CC guard columns**:		8 x 3 mm	761557.30		8 x 4 mm	761557.40
NUCLEODUR® Sphinx RP, 5 µm
2 mm ID		760800.20		760809.20	760801.20	760802.20	760803.20
3 mm ID		760800.30		760809.30	760801.30	760802.30	760803.30
4 mm ID		760800.40		760809.40	760801.40	760802.40	760803.40
4.6 mm ID		760800.46	760815.46	760809.46	760801.46	760802.46	760803.46
EC guard columns*:		4 x 2 mm	761922.20		4 x 3 mm	761922.30
CC guard columns**:		8 x 3 mm	761550.30		8 x 4 mm	761550.40
Guard columns in packs of 3, EC columns in packs of 1

Guard columns for EC columns			EC columns with ID				Use guard column holder
			2 mm	3 mm	4 mm	4.6 mm	Use guard column holder
*	Column Protection System	EC guard column	4/2	4/3	4/3	4/3	REF 718966
**	ChromCart® guard column system	CC guard column	8/3	8/3	8/4	8/4	REF 721359
Custom-packed EC columns with other dimensions are available on request.
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Microbore columns for analytical HPLC
Ordering information
Eluent in columns acetonitrile – water

Length ->	50 mm	100 mm	125 mm	150 mm	250 mm
NUCLEODUR® Sphinx RP, 3 µm
1 mm ID	717686.10	717685.10		717687.10
NUCLEODUR® Sphinx RP, 5 µm
1 mm ID	717680.10	717681.10	717682.10	717683.10	717684.10
All columns in packs of 1. Guard columns for microbore columns on request!

On request, Microbore columns are also available in lengths of 40, 60, 200, and 300 mm and with 0.05 · 0.075 · 0.1 · 0.15 · 0.3 · 0.4 · 0.5 · 0.75 and 1.5 mm ID.

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VarioPrep columns for preparative HPLC
Ordering information
Eluent in column acetonitrile – water

Length	10 mm ID	21 mm ID	32 mm ID	40 mm ID
NUCLEODUR® Sphinx RP, 5 µm
50 mm	762372.100	762372.210
125 mm	762375.100	762375.210
150 mm				762371.400
250 mm	762373.100	762373.210	762373.320	762373.400
VP guard columns with NUCLEODUR® Sphinx RP
	8 mm ID	16 mm ID	32 mm ID
10 mm*	762390.80	762390.160
15 mm*			762392.320

* VP guard column	Size	Pack of	for VP column	required holder	REF
VP 10/8	10 x 8 mm ID	2	10 mm ID	8 mm	718251
VP 10/16	10 x 16 mm ID	2	21 mm ID	16 mm	718256
VP 15/32	15 x 32 mm ID	1	32–40 mm ID	32 mm	718253
Custom-packed VP columns with other dimensions are available on request.
For details of the VarioPrep column system click here.
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