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Instruction leaflet:
NUCLEODUR®
RP columns


  
   
NUCLEODUR® C18 Pyramid
High purity silica phase for HPLC
Octadecyl modification with polar endcapping,
suitable for aqueous eluents · endcapped · 14 % C · USP L1
Properties
Key features:
Stable in 100 % aqueous mobile phase systems
Interesting polar selectivity features
Excellent base deactivation; suitable for LC/MS due to low bleeding characteristics
Technical characteristics:
Special phase with polar endcapping
Pore size 110 Å; particle sizes 1.8 μm, 3 μm and 5 μm
7 and 10 μm particles for preparative purposes on request
Carbon content 14 %; pH stability 1–9
Recommended application:
Analgesics, penicillin antibiotics, nucleic acid bases, water-soluble vitamins, complexing agents, organic acids
For applications click here.


Standard program* of columns packed with NUCLEODUR® C18 Pyramid
Column system Particle size: 1.8 µm 3 µm 5 µm
Analytical EC columns (standard system, 2–4.6 mm)
  Microbore columns (MB, 1 mm)  
Preparative VarioPrep columns (VP, ≥ 8 mm)    
if you do not know our HPLC column systems, click the "i" for further information
* Please note! Most combinations of MN column types, available column dimensions and particle sizes can be custom-packed on request.

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EC standard columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water
Length -> 30 mm 50 mm 75 mm 100 mm 125 mm 150 mm 250 mm
NUCLEODUR® C18 Pyramid, 1.8 µm
2 mm ID 760271.20 760272.20 760275.20 760273.20   760274.20  
3 mm ID 760271.30 760272.30   760273.30      
4 mm ID 760271.40 760272.40   760273.40      
4.6 mm ID 760271.46 760272.46   760273.46      
EC guard columns*: 4 x 2 mm 761915.20   4 x 3 mm 761915.30  
NUCLEODUR® C18 Pyramid, 3 µm
2 mm ID   760263.20   760264.20 760260.20 760261.20 760262.20
3 mm ID   760263.30   760264.30 760260.30 760261.30 760262.30
4 mm ID   760263.40   760264.40 760260.40 760261.40 760262.40
4.6 mm ID   760263.46 760259.46 760264.46 760260.46 760261.46 760262.46
EC guard columns*: 4 x 2 mm 761916.20   4 x 3 mm 761916.30  
CC guard columns**: 8 x 3 mm 761854.30   8 x 4 mm 761854.40  
NUCLEODUR® C18 Pyramid, 5 µm
2 mm ID   760200.20   760204.20 760201.20 760203.20 760202.20
3 mm ID   760200.30   760204.30 760201.30 760203.30 760202.30
4 mm ID   760200.40   760204.40 760201.40 760203.40 760202.40
4.6 mm ID   760200.46 760205.46 760204.46 760201.46 760203.46 760202.46
EC guard columns*: 4 x 2 mm 761917.20   4 x 3 mm 761917.30  
CC guard columns**: 8 x 3 mm 761800.30   8 x 4 mm 761800.40  
Guard columns in packs of 3, EC columns in packs of 1

Guard columns for EC columns   EC columns with ID: Use guard
column holder
      2 mm 3 mm 4 mm 4.6 mm
* Column Protection System EC guard column 4/2 4/3 4/3 4/3 REF 718966
** ChromCart® guard column system CC guard column 8/3 8/3 8/4 8/4 REF 721359
Custom-packed EC columns with other dimensions are available on request.
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Microbore columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water
Length -> 50 mm 100 mm 125 mm 150 mm 250 mm
NUCLEODUR® C18 Pyramid, 3 µm
1 mm ID 717740.10 717741.10 717742.10 717743.10 717744.10
NUCLEODUR® C18 Pyramid, 5 µm
1 mm ID   717722.10 717723.10 717724.10 717725.10
All columns in packs of 1. Guard columns for microbore columns on request!
On request, Microbore columns are also available in lengths of 40, 60, 200, and 300 mm and with 0.05 · 0.075 · 0.1 · 0.15 · 0.3 · 0.4 · 0.5 · 0.75 and 1.5 mm ID.
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VarioPrep columns for preparative HPLC
Ordering information
Eluent in column acetonitrile – water
Length 10 mm ID 21 mm ID 32 mm ID 40 mm ID
NUCLEODUR® C18 Pyramid, 5 µm
50 mm 762271.100 762271.210    
125 mm 762273.100 762273.210    
150 mm       762269.400
250 mm 762272.100 762272.210 762272.320 762272.400
VP guard columns with NUCLEODUR® C18 Pyramid
  8 mm ID 16 mm ID 32 mm ID
10 mm* 762291.80 762291.160    
15 mm*     762293.320
* VP guard column Size Pack of for VP column required holder REF
VP 10/8 10 x 8 mm ID 2 10 mm ID 8 mm 718251
VP 10/16 10 x 16 mm ID 2 21 mm ID 16 mm 718256
VP 15/32 15 x 32 mm ID 1 32–40 mm ID 32 mm 718253
Custom-packed VP columns with other dimensions are available on request.
For details of the VarioPrep column system click here.
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