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Instruction leaflet:
NUCLEODUR® HILIC

  
   
NUCLEODUR® HILIC
High purity silica phase for
Hydrophilic Interaction Chromatography
Properties
Key features:
Ideal for reproducible and stable chromatography of highly polar analytes
Suitable for analytical and preparative applications as well as LC-MS
Very short column conditioning period
Technical characteristics:
Ammonium – sulfonic acid modified silica: pore size 110 Å, available particle sizes 1.8 µm, 3 µm and 5 µm; carbon content 7 %;
pH stability 2–8.5
Recommended application:
Hydrophilic compounds such as organic polar acids and bases, polar natural compounds, nucleosides, oligonucleotides, amino acids, peptides, water soluble vitamins
For further applications click here.


Standard program* of columns packed with NUCLEODUR® HILIC
Column system Particle size: 1.8 µm 3 µm 5 µm  
Analytical EC columns (standard system, 2–4.6 mm)  
  if you do not know our HPLC column systems, click the "i" for further information
* Please note! Most combinations of MN column types, available dimensions and particle sizes can be custom-packed on request.

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EC standard columns for analytical HPLC
Ordering information
Eluent in column acetonitrile – water (80:20, v/v)
Length-> 30 mm 50 mm 75 mm 100 mm 125 mm 150 mm 250 mm
NUCLEODUR® HILIC, 1.8 µm
2 mm ID 760521.20 760523.20 760525.20 760526.20   760528.20  
3 mm ID 760521.30 760523.30   760526.30      
4 mm ID 760521.40 760523.40   760526.40      
4.6 mm ID 760521.46 760523.46   760526.46      
EC guard columns*   4 x 2 mm 761960.20   4 x 3 mm 761960.30  
NUCLEODUR® HILIC, 3 µm
2 mm ID   760532.20   760534.20 760531.20 760533.20 760530.20
3 mm ID   760532.30   760534.30 760531.30 760533.30 760530.30
4 mm ID   760532.40   760534.40 760531.40 760533.40 760530.40
4.6 mm ID   760532.46   760534.46 760531.46 760533.46 760530.46
EC guard columns*   4 x 2 mm 761961.20   4 x 3 mm 761961.30  
CC guard columns**   8 x 3 mm 761580.30   8 x 4 mm 761580.40  
NUCLEODUR® HILIC, 5 µm
2 mm ID   760552.20   760554.20 760551.20 760553.20 760550.20
3 mm ID   760552.30   760554.30 760551.30 760553.30 760550.30
4 mm ID   760552.40   760554.40 760551.40 760553.40 760550.40
4.6 mm ID   760552.46   760554.46 760551.46 760553.46 760550.46
EC guard columns*   4 x 2 mm 761962.20   4 x 3 mm 761962.30  
CC guard columns**   8 x 3 mm 760590.30   8 x 4 mm 761590.40  
Guard columns in packs of 3, columns in packs of 1

Guard columns for EC columns   EC columns with ID Use guard
column holder
      2 mm 3 mm 4 mm 4.6 mm
* Column Protection System EC guard column 4/2 4/3 4/3 4/3 REF 718966
** ChromCart® guard column system CC guard column 8/3 8/3 8/4 8/4 REF 721359
Custom-packed EC columns with other dimensions are available on request.
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